Study of the effect of protein synthesis inhibitors on growing <i>Escherichia coli</i> bacteria using electrochemical sensors

Background. The study of the mechanisms action antibiotics requires integrated use traditional microbiological and physicochemical methods. aim. To response Escherichia coli bacteria to four antibiotics, inhibitors protein synthesis, using combined approach. Methods. Bacteria were grown under aerobic conditions on minimal M9 medium with glucose. Tetracycline, kanamycin, streptomycin chloramphenicol have been tested. effect survival (CFU) growth rate was determined. Respiratory activity, sulfide production, extracellular potassium, as well pH Eh measured electrochemical sensors directly in a growing culture “real time”, membrane potential DiBAC dye Leica DM2000 fluorescent microscope. Results. tested divided into two groups according their properties. Tetracycline showed pronounced bacteriostatic effect, inhibition began immediately after addition occurred at high rate. Both inhibited respiration, stimulated production an jump. treated tetracycline retained ability maintain intracellular potassium better. Inhibition respiration led decrease glucose catabolism, evidenced by lower acidification compared control. Growth kanamycin initiated 30-minute delay. bactericidal did not stimulate jump, inhibit but caused drop potassium. High respiratory activity promoted rapid medium. Of interest is detection kanamycin-induced during E. MOPS Conclusion. An analysis data obtained indicates that combination methods promising approach studying antibiotics.